high content screening rnai

iScreen: Image-Based High-Content RNAi Screening

Recently, advanced image technologies have been coupled with high-throughput RNAi screening to develop high-content screening, in which one or more cell image(s), instead of a single readout, were generated from each well. This image-based high-content screening technology has led to genome-wide functional annotation in a wider spectrum of biological research studies, as well as in drug and ...

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High-content genome-wide RNAi screens identify

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy Nature. 2013 Dec 12;504(7479) :291-5. doi ... screens coupled to high-content microscopy. Screening yielded gene candidates involved in diverse cellular processes that were subsequently validated in low-throughput assays. This led to characterization of TOMM7 as essential for stabilizing PINK1 on the ...

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A high-content RNAi screen reveals multiple roles for long ...

15/04/2020  With a high-content RNAi imaging screen targeting more than 2,000 human lncRNAs, we identify numerous lncRNAs involved in key steps of cell division such as

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High-content genome-wide RNAi screens identify

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy Nature. 2013 Dec 12;504(7479) :291-5. doi ... screens coupled to high-content microscopy. Screening yielded gene candidates involved in diverse cellular processes that were subsequently validated in low-throughput assays. This led to characterization of TOMM7 as essential for stabilizing PINK1 on the ...

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iScreen: Image-Based High-Content RNAi Screening

Recently, advanced image technologies have been coupled with high-throughput RNAi screening to develop high-content screening, in which one or more cell image(s), instead of a single readout, were generated from each well. This image-based high-content screening technology has led to genome-wide functional annotation in a wider spectrum of biological research studies, as well as in drug and ...

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High-throughput, High Content RNAi Screening

RNAi screening becomes even more powerful when combined with high content imaging and data analysis techniques as the resulting multiparametric readouts provide greater insight toward the understanding of gene function. For an academic customer, we built a fully integrated RNAi screening system to be deployed in a space restricted environment. Inside the compact explorer

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High Content Screening meets RNAi - Thermo Fisher

High Content Screening meets RNAi •What is HCS? •Reagents, Software, Hardware •Why should I use it? •What advantage does it bring to RNAi •How can it be used? •Real life examples. 3 CONFIDENTIAL PROPRIETARY High Content Screening/High Content Analysis • High Content Screening is an Integration of: •Cell-based assays •Robotics •High resolution fluorescence microscopy ...

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High-content genome-wide RNAi screens identify

24/11/2013  Mitophagy is the elimination of damaged mitochondria by the autophagosome regulated by the ubiquitin ligase, parkin and the kinase PINK1; a genome-wide RNAi screen with high-content microscopy has ...

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iScreen: Image-Based High-Content RNAi Screening

High-throughput RNA interference (RNAi) screening has opened up a path to investigating functional genomics in a genome-wide pattern. However, such studies are often restricted to assays that have ...

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High Content Screening SpringerLink

High-Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR, and Small Molecule Screening. Steven A. Haney. Pages 113-130. Strategies and Solutions to Maintain and Retain Data from High Content Imaging, Analysis, and Screening Assays . K. Kozak, B. Rinn, O. Leven, M. Emmenlauer. Pages 131-148. Live-Cell High Content Screening in Drug Development. Milan Esner,

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A Decade of RNAi Screening: Too much hay and very few ...

22/08/2013  12 Falschlehner, C et al. High-throughput RNAi screening to dissect cellular pathways: a how-to guide. Biotechnol J 5, 368-76 (2010). ... A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Cell 124, 1283-98 (2006). 60 Chang, K et al. Lessons from nature: microRNAbased shRNA libraries. Nat Methods 3, 707-14 (2006). 61 Gu, S et al. The loop ...

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RNA fluorescence in situ hybridization for high-content ...

15/08/2017  High Content Screening microscopes which are available with high magnification objectives (60X or 100X) appropriate for RNA FISH screening are the IN Cell Analyser 6000 (GE Healthcare, Issaquah, WA, United States), the Opera and Opera Phenix systems (PerkinElmer, Waltham, MA, United States), and the ImageXpress Micro Confocal and Micro 4 platforms (Molecular Devices,

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High-Content Screening and Analysis PerkinElmer

High-Content Analysis (HCA) or High-Content Screening (HCS) combines high-throughput automated imaging and analysis to extract quantitative multi-parametric data at the single-cell level. Originally developed as a complementary technology to traditional biochemical high-throughput screening (HTS) in drug discovery, today high-content screening is established in a far broader area of the life ...

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High Content Screening meets RNAi - Thermo Fisher

High Content Screening meets RNAi •What is HCS? •Reagents, Software, Hardware •Why should I use it? •What advantage does it bring to RNAi •How can it be used? •Real life examples. 3 CONFIDENTIAL PROPRIETARY High Content Screening/High Content Analysis • High Content Screening is an Integration of: •Cell-based assays •Robotics •High resolution fluorescence microscopy ...

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High-content genome-wide RNAi screens identify

24/11/2013  Mitophagy is the elimination of damaged mitochondria by the autophagosome regulated by the ubiquitin ligase, parkin and the kinase PINK1; a genome-wide RNAi screen with high-content microscopy has ...

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iScreen: Image-Based High-Content RNAi Screening

High-throughput RNA interference (RNAi) screening has opened up a path to investigating functional genomics in a genome-wide pattern. However, such studies are often restricted to assays that have ...

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IScreen: Image-Based High-Content RNAi Screening

Recently, advanced image technologies have been coupled with high-throughput RNAi screening to develop high-content screening, in which one or more cell image(s), instead of a single readout, were generated from each well. This image-based high-content screening technology has led to genome-wide functional annotation in a wider spectrum of biological research studies, as well as in drug and ...

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High-Content Chemical and RNAi Screens for Suppressors of ...

High-Content Chemical and RNAi Screens for Suppressors of Neurotoxicity in a Huntington’s Disease Model Joost Schulte1*, Katharine J. Sepp1, Chaohong Wu2, Pengyu Hong2, J. Troy Littleton1 1Department of Biology, Department of Brain and Cognitive Sciences, The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge,

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Automated microscopy for high-content RNAi screening ...

iScreen: Image-Based High-Content RNAi Screening Analysis Tools. Zhong R, Dong X, Levine B, Xie Y, Xiao G. J Biomol Screen, 20(8):998-1002, 29 Dec 2014 Cited by: 3 articles PMID: 25548139. A Perspective on the Future of High-Throughput RNAi Screening: Will CRISPR Cut Out the Competition or Can RNAi Help Guide the Way? Taylor J, Woodcock S. J Biomol Screen, 20(8):1040-1051, 05 Jun

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High Content Screening SpringerLink

High-Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR, and Small Molecule Screening. Steven A. Haney. Pages 113-130. Strategies and Solutions to Maintain and Retain Data from High Content Imaging, Analysis, and Screening Assays . K. Kozak, B. Rinn, O. Leven, M. Emmenlauer. Pages 131-148. Live-Cell High Content Screening in Drug Development. Milan Esner,

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RNAi library and high-content screening deal

RNAi library and high-content screening deal. 9 Aug 2005 by Xantos Biomedicine. Share: Xantos Biomedicine and Qiagen announce a co-marketing agreement for genome-wide RNAi library with high-content cellular screening system. Xantos Biomedicine and Qiagen have announced a partnership for co-marketing Qiagen's proprietary Tom-amidite chemistry based genome-wide RNAi products with

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RNA fluorescence in situ hybridization for high-content ...

15/08/2017  High Content Screening microscopes which are available with high magnification objectives (60X or 100X) appropriate for RNA FISH screening are the IN Cell Analyser 6000 (GE Healthcare, Issaquah, WA, United States), the Opera and Opera Phenix systems (PerkinElmer, Waltham, MA, United States), and the ImageXpress Micro Confocal and Micro 4 platforms (Molecular Devices,

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RNA interference - Wikipedia

Genome-scale RNAi research relies on high-throughput screening (HTS) technology. RNAi HTS technology allows genome-wide loss-of-function screening and is broadly used in the identification of genes associated with specific phenotypes. This technology has been hailed as a potential second genomics wave, following the first genomics wave of gene expression microarray and single

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